All Names:

5'-AMP-activated protein kinase, catalytic alpha-1 chain, AAPK1, AMPK alpha-1 chain, AMPK-alpha1, EC 2.7.11.1, HMG-CoA redustase kinase, PRKAA1, acetyl-CoA carboxylase kinase, hormone-sensitive lipase kinase, kinase AMPK-alpha1

All Sites:

Human: Thr172; Mouse: Thr172; Rat: Thr172

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Store at -20℃/1 year

Immunogen:

The antiserum was produced against synthesized phosphopeptide derived from humanAMPKα around the phosphorylation site of threonine172(L-R-T^P-S-C).

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificity/Sensitivity:

AMPKα (Phospho-Thr172) antibodydetects endogenous levels of AMPKα only when phosphorylated at threonine172 in both chain 1 and 2.

Reactivity:

Human, Mouse, Rat

Applications:

WB: 1:500~1:3000      IHC: 1:50~1:100

ELISA: 1:40000